Analysis of Induced Mutants of Salinity Resistant Banana (Musa acuminata cv. Dwarf Cavendish) Using Morphological and Molecular Markers

Authors

  • Ali Reza Talaei Department of Horticulture, Faculty of Agriculture, Islamic Azad University-Science and Research Branch, P.O. Box 14515-775, Tehran, I.R. Iran
  • Amir Mousavi Department of Plant Biotechnology, National Institute of Genetic Engineering and Biotechnology, P.O. Box 14965/161, Tehran, I.R. Iran
  • Behnam Naserian Khiabani gricultural, Medical and Industrial Research School, Atomic Energy Organization of Iran, P.O. Box 31485-498, Karaj, I.R. Iran
  • Mohammad Mirzaii gricultural, Medical and Industrial Research School, Atomic Energy Organization of Iran, P.O. Box 31485-498, Karaj, I.R. Iran
  • Mohammad Reza Naghavi Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Tehran, P.O. Box 31587-11167, Karaj, I.R. Iran
  • Seied Mehdi Miri Department of Horticulture, Faculty of Agriculture, Islamic Azad University-Science and Research Branch, P.O. Box 14515-775, Tehran, I.R. Iran
Abstract:

Genetic diversity amongst 21 induced mutant clones tolerant to salinity along with one non-irradiated sensitive clone of banana (Musa acuminata cv. Dwarf Cavendish (AAA)) were studied using morphological and random amplified polymorphic DNA (RAPD) markers. Out of the 30 phenotypic indices screened, 23 were polymorph and two traits, leaf habit and blotches color, were differentiated by non-irradiated clone. RAPDs established 106 major amplified products using 14 primers. Out of 106 markers, eight were monomorph, and the remaining (98) were polymorph. The extent of polymorphism indicated the existence of considerable variation DNA level within induced mutant clones. Primer OPA-02 revealed banding patterns specific to salinity resistant clones. Both morphological and RAPD analyses successfully detected genetic variation within induced mutant clones, RAPD also detected variation between the irradiated and non-irradiated clones, which were morphologically indistinguishable. Results were indicative that induced mutations bear a great potential in improving banana for salinity resistant.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

analysis of induced mutants of salinity resistant banana (musa acuminata cv. dwarf cavendish) using morphological and molecular markers

genetic diversity amongst 21 induced mutant clones tolerant to salinity along with one non-irradiated sensitive clone of banana (musa acuminata cv. dwarf cavendish (aaa)) were studied using morphological and random amplified polymorphic dna (rapd) markers. out of the 30 phenotypic indices screened, 23 were polymorph and two traits, leaf habit and blotches color, were differentiated by non-irrad...

full text

Malate synthase gene expression during fruit ripening of Cavendish banana (Musa acuminata cv. Williams).

Malate synthase (MS) is a key enzyme responsible for malic acid synthesis in the glyoxylate cycle, which functions to convert stored lipids to carbohydrates, by catalysing the glyoxylate condensation reaction with acetyl-CoA in the peroxisome. In this study, the cloning of an MS cDNA, designated MaMS-1, from the banana fruit is reported. MaMS-1 was 1801 bp in length encoding a single polypeptid...

full text

Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan

MS (Murashige and Skoog) media supplemented with benzylaminopurine (BAP) showed that the number of bud formation in shoot cultures of Musa acuminata cv.Berangan during the initiation stage increased proportionately with the concentrations used (11, 22 and 33 μM). However, the highest concentration of BAP (33 μM) simultaneously increased the formation of abnormal shoots. After the first apical b...

full text

Monoxenic Culture of Banana-Parasitic Nematodes on Musa acuminata cv. Poyo shoots.

Among nematodes parasitizing bananas throughout the world, Radopholus similis and Helicotylenchus multicinctus are the most widespread and damaging species. Besides these two nematodes, Hoplolaimus pararobustus is encountered frequently in Ivory Coast and has been increasing in banana growing areas for the past 25 years (6,10). In vitro culture systems could facilitate screening for resistance ...

full text

Carbohydrate binding properties of banana (Musa acuminata) lectin

Examination of lectins of banana (Musa acuminata) and the closely related plantain (Musa spp.) by the techniques of quantitative precipitation, hapten inhibition of precipitation, and isothermal titration calorimetry showed that they are mannose/glucose binding proteins with a preference for the a-anomeric form of these sugars. Both generate precipitin curves with branched chain a-mannans (yeas...

full text

My Resources

Save resource for easier access later

Save to my library Already added to my library

{@ msg_add @}


Journal title

volume 7  issue 2

pages  86- 92

publication date 2009-04-01

By following a journal you will be notified via email when a new issue of this journal is published.

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023